2015-2016
North Carolina Department of Agriculture
& Consumer Services
Veterinary Division
Steven W. Troxler, Commissioner of Agriculture
R. Douglas Meckes, DVM, State Veterinarian
James Trybus, DVM, DACVP, Director of Laboratories
NCVDLS Annual Report
Volume 5
2/20/2017
North Carolina Veterinary Diagnostic Laboratory System
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Table of Contents
Page
2 Mission Statement/Message from the Director
3-6 Facilities and Laboratory Personnel
7-8 External Laboratory Advisory Committee
9 Budget and Facility Information
10 Improvements, New Equipment & New Test Procedures
10 Professional Development and Outreach
11 Proficiency Testing
12-14 NCVDLS Accession Information
Rollins Animal Disease
Diagnostic Laboratory
2101 Blue Ridge Road
Raleigh, NC 27607
(919) 733-3986
Western Animal Disease
Diagnostic Laboratory
785 Airport Road
Fletcher, NC 28732
(828) 684-8188
Northwestern Animal Disease
Diagnostic Laboratory
1689 N. Bridge St.
Elkin, NC 28621
(336) 526-2499
Griffin Animal Disease
Diagnostic Laboratory
401 Quarry Rd.
Monroe, NC 28112
(704) 289-6448
NCVDLS Facilities
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2
On an annual basis, we assemble summaries of the activities within the
North Carolina Veterinary Diagnostic Laboratory System (NCVDLS). The
information contained within is for the period between July 1, 2015 and June 30,
2016. Data from all four laboratories comprising our system are included.
Services have been on the increase, with an escalation of 5.33% overall in
laboratory accessions when compared to the previous fiscal year. We hope you
find this information to be both interesting and useful. Our goal, as always, is to
provide clients with accurate and timely service to meet our Mission, as stated
above. Please do not hesitate to contact me about this annual report, if you have
questions.
Respectfully yours,
James Trybus, DVM, D-ACVP
NCVDLS Mission Statement
It is the mission of the North Carolina Veterinary Diagnostic
Laboratory System to provide veterinarians, the animal industry,
and the citizens of North Carolina with accurate and timely
laboratory support services in order to diagnose, conduct
surveillance, and assist in responding to and preventing animal
disease. Both protection of public health and the food supply are
Important components of this mission.
3
NCVDLS Facilities and Laboratory Personnel
Rollins Animal Disease Diagnostic Laboratory
1031 Mail Service Center
Raleigh, NC 27699-1031
Phone: (919)733-3986
Fax: (919)733-0454
Director of Laboratories
James Trybus DVM, D-ACVP
Assistant Director
Richard Mock, PhD
Administrative Staff
Cheryl Hearn Administrative Officer I
Mechelle Johnson Administrative Assistant I
Rebecca Boone Processing Assistant IV
Carol Garbarino Processing Assistant IV
Paul Miller Processing Assistant III
Cindy Orlosky Processing Assistant V
Brandy Rodgers Processing Assistant IV
Tamara Seago Processing Assistant V
Dominic Gonzalez Medical Laboratory Assistant II
Dell Weaver Medical Laboratory Assistant II
Bacteriology
Sandy Murphy, BS, M-ASCP Medical Laboratory Supervisor I, Section Supervisor
Melissa Cox Medical Laboratory Technologist I
Lee Goldbach, MS Medical Laboratory Technologist I
Megan Flowers, BS Medical Laboratory Technician II
Richard Koch Medical Laboratory Technologist I
Elizabeth Ortiz, BS Medical Laboratory Technologist II
Bill O’Shields Medical Laboratory Assistant III
Jeri Smart, BS, Medical Laboratory Technologist I
Sommer Souther, BS Medical Laboratory Technologist I
Laura Tweed Medical Laboratory Technologist I
Building Maintenance
Stephen Szymczyk Maintenance Mechanic V, Section Supervisor
Joey Stephenson Maintenance Mechanic IV
Information Technology
Josh Shepherd Business and Technology Applications Analyst, Section Supervisor
Talley Ouro Business and Technology Applications Analyst
Molecular Diagnostics
Beverly Wood, BS, MT-ASCP Laboratory Medical Specialist, Section Supervisor
Neeti Dahal, BS, PhD Medical Laboratory Technologist II
Younghee Lee, MS, BS Medical Laboratory Technologist II
Chad Menard, BS Medical Laboratory Technologist III
Ashlee Zack, BS, Medical Laboratory Technologist II
Pathology Services Coordinator
James Trybus, DVM, D-ACVP
Avian Pathologist
Tahseen Abdul Aziz, DVM, MS, PhD, D-ACPV
4
Anatomic Pathologists
Allison Boone, DVM, D-ACVP
Steve Rushton, DVM, D-ACVP
Alison Tucker, MS, VMD, D-ACVP
Diagnosticians
Mahogany Caesar, MS, DVM
Jennifer Haugland, DVM
Stacy Robinson, DVM
Necropsy Assistant
Jennifer Burgoyne, BS Medical Laboratory Technician II
Histopathology
Mary Baker, HT-ASCP Medical Laboratory Technologist III, Section Supervisor
Marcia Sutton, ASGE Medical Laboratory Technologist I
Judy Liverman Medical Laboratory Technician II
Cindy Nipper, HT-ACSP Medical Laboratory Technologist I
Immunohistochemistry
David Weil, BS, HT-ASCP Medical Laboratory Technologist I
Quality Assurance and Safety
Tina Buffington, BS Medical Laboratory Supervisor III, Quality Manager
Ghazala Jawad, MS Medical Laboratory Supervisor III, Quality Manager
Connie Howen Processing Assistant IV
Paul Hutter, BS Safety Officer III, Division Safety Officer
Serology
Kristen Crook, BS, PhD Medical Laboratory Technologist III, Section Supervisor
Brandon Brown, BS Medical Laboratory Technologist I
Kim Gooden Medical Laboratory Technologist I
Aisha Phipps, BS Medical Laboratory Technologist I
Virology
Chad Cecil BS, PhD Medical Laboratory Supervisor III, Section Supervisor
Jonathan Fox, BS Medical Laboratory Technician II
Kim Howle, BA, MS Medical Laboratory Technologist III
Fuhing Lo, BS Medical Laboratory Technician II
Angie Murphy, BS Medical Laboratory Technologist II
Excel Swann, BS Medical Laboratory Technologist I
Jenna Hunter, BS Medical Laboratory Technologist I
Amy Nipper, BS Medical Laboratory Technologist I
Carla Spears, BS Medical Laboratory Technologist I
5
NCVDLS Branch Facilities
Griffin Animal Disease Diagnostic Laboratory
401 Quarry Road
Monroe, NC 28112
Phone: (704)289-6448
Fax: (704)283-9660
Northwestern Animal Disease Diagnostic Laboratory
1689 N. Bridge St.
Elkin, NC 28621
Phone: (336)526-2499
Fax: (336)526-2603
David Ackerman, Resident Director
Jessica Kees, DVM Veterinary Diagnostician
Barbara Bright, BS Medical Laboratory Technologist II
Marcella Galyean, AAS Medical Laboratory Technician II
Dana Kelly Veterinary Laboratory Assistant I
Cindy Smith Processing Assistant IV
Western Animal Disease Diagnostic Laboratory
785 Airport Road
Fletcher, NC 28732
Phone: (828)684-8188
Fax: (828)687-3574
Richard Oliver, DVM Resident Director
David Drum, DVM, MS Veterinary Diagnostician
Darlene Ayers Processing Assistant IV
Lia Barth, BS Medical Laboratory Technician II
Wilson Smith Medical Laboratory Assistant II
Mary Swanson, DVM Medical Laboratory Technician II
Kim Hagans, DVM Resident Director
Reg Ridenhour, DVM Veterinary Diagnostician
Sherry Casey Processing Assistant IV
Kathy Harwood, AAS Medical Laboratory Technologist I
Jimmy Walters Medical Laboratory Technician I
Carrie Lichtenhan, BS Medical Laboratory Technician II
Kristie Long Medical Laboratory Technician II
6
SERVICE MILESTONES, 2015-2016
Dr. Mary Swanson 10
Cindy Orlosky 10
Dr. Taheen Aziz 10
Dr. Stacy Robinson 15
Kristie Long 20
Kathy Harwood 25
Kim Gooden 30
Paul Miller 30
RETIREMENTS
Helen Tang Rollins Laboratory Virology 12/1/2015
Fuhing Lo Rollins Laboratory Virology 3/1/2016
William O’Shields Rollins Laboratory Bacteriology 10/1/2015
Dr. Karen Post Director 12/1/2015
External Laboratory Advisory Committee Members
The NCVDLS established an External Laboratory Advisory Committee (ELAC) in 2005. Our
initial purpose was to better serve our clients by uniting a diverse group of veterinarians and
industry representatives to discuss the laboratory’s offered services, as well as legislation that
directly affected our mission. We are appreciative of their time and advice.
NAME EMAIL ADDRESS
Deborah Johnson, Executive Director
NC Pork Council
deborah@ncpork.rog
Dr. Eric Gonder, Veterinarian
Butterball
egonder@butterball.com
Dr. Allen Cannedy
Small Ruminant Veterinarian
goat-doc@mymobilevet.com
Sue Gray, Executive Director
NC Horse Council
suegray@nchorsecouncil.com
Dr. Randy Jones
Mixed Animal Veterinarian
Swine Practice, Kinston, NC
rjones@livestockvet.com
Harry Snelson,, Executive Director
American Association of Swine Veterinarians
snelson@aasv.org
Bryan Blinson, Executive Director
NC Cattlemen
bryan@nccattle.com
Don Butler, Director of Government Relations &
Public Affairs
Murphy Brown
dpbutler@smithfield.com
Dr. Becky Tilley, Veterinarian
Butterball
btilley@butterball.com
Dr. Betsy Sigmon, Veterinarian
Small Animal Practice
btsigmon@aol.com
Dr. Chad Smith, Veterinarian
Swine Practice, Goldsboro Milling
chad.smith@goldsboromilling.com
Dr. Paula Cray, Department Head
Population Health & Pathobiology, NCSUCVM
pjcray@ncsu.edu
Dr. Richard Kirkman, Veterinarian
Large Animal, Siler City, NC
rkirkman@combuy.com
8
External Laboratory Advisory Committee Members cont’
NAME EMAIL ADDRESS
Dr. Shannon Jennings, Veterinarian
Poultry, House of Raeford
shannon.jennings@houseofraeford.com
Dr. Jennifer Haugland, Veterinarian Diagnostician
NCDA&CS, Rollins Animal Disease Diagnostic
Laboratory
jennifer.haugland@ncagr.gov
Norman Jordan, Past President
NC Dairy Producers Association
njordan7483@gmail.com
Reid Smith, Current President
NC Dairy Producers Association
reid@linwooddairygroup.com
Dr. Douglas Meckes, State Veterinarian
NCDA&CS, Veterinary Division
doug.meckes@ncagr.gov
Dr. James Trybus, Director of Laboratories
NCDA&CS, Veterinary Division
james.trybus@ncagr.gov
Bob Ford, Executive Director
Poultry Federation
rlford@ncpoultry.org
Dr. Glen Almond, Veterinarian
NCSUCVM
gwalmond@ncsu.edu
Dr. Perry Harms, Veterinarian
Murphy Brown
pharms@smithfield.com
Kent Yelverton, Division Director
NCDA&CS, Property & Construction
kent.yelverton@ncagr.gov
Megan Jacob, Laboratory Director
NCSUCVM
megan_jacob@ncsu.edu
Chester Lowder, Livestock Director
Farm Bureau
chester.lowder@ncfb.org
Jan Kelly, Executive Director
NC Egg Association
egglady@ncegg.org
Dr. Rich Sharpton, Veterinarian
Poultry, Perdue Milling
rick.sharpton@perdue.com
9
General Funds, Funded Contracts and Cooperative Agreements
2015-2016 NCVDLS General Funds
2015/2016 Recurring Cooperative or Blanket Purchase Agreements
USDA-APHIS-VS Blanket Purchase Agreement—$24,440
 reimbursement for BSE Surveillance Sample Collection and Testing
USDA-APHIS-VS Blanket Purchase Agreement—$6,480
 reimbursement for Scrapie Sample Collection and Testing
USDA-AHPIS-VS Swine Surveillance Cooperative Agreement—$26,500
 reimbursement for surveillance and testing of Classical Swine Fever and PRV samples
USDA-NIFA Cooperative Agreement—$326,000
 to aid the Food and Agriculture Defense Initiative for surveillance and early
detection of animal diseases
Budget Summary 2014/2015 2015/2016
Purchased Contractual Services 5321 $174,526 $184,424
Utility/Energy Services 5322 $303,378 $264,502
Repair Services 5323 $101,969 $105,843
Maintenance Agreements 5324 $87,921 $140,438
Rentals/Leases 5325 $6,323 $5,175
Travel & Other Employee Expenses 5327 $39,217 $43,741
Communications & Data 5328 $99,574 $108,555
Other Services 5329 $14,602 $27,255
General Admin Supplies 5331 $38,141 $28,623
Facility and Hardware Supplies 5332 $28,032 $23,522
Vehicle/Equipment Operating Supplies 5333 $1,748 $1,804
Clothing and Recreational Supplies 5335 $730 $1,306
Research/Development & Edu Supplies 5337 $1,248,477 $1,014,532
Other Materials & Supplies 5339 $5,811 $13,354
Equipment 5345 $107,264 $323,782
Art, Other Artifacts & Literature 5346 $1,822 $2,052
Intangible Assets (Computer software) 5347 $3,131 $44,462
Legal, Licenses & Permit Costs 5351 $6,715 $6,182
Other Admin Expenses 5358 $4,867 $4,357
Other Expenses 5359 $4,029 $8,675
Transfer of Appropriations 5389 $80,281 $0
TOTAL $2,358,558 $2,352,584
Personal Services (Salaries, Benefits, etc.) $5,257,814 $5,324,958
TOTAL $7,616,371 $7,677,542
10
2015-2016 Physical Improvements/New Equipment Purchases
Rollins Laboratory
 Molecular Diagnostics—Smart Cycler Desktop Computer, Ergonomic Pipet, Refrigerator/Freezer, 3
Door Cooler, Fax/Printer/Scanner with Computer, ABI 7510 Fast, Mini Centrifuge
 Serology—BioTek plate washer, Electronic Multichannel Pipet, Manual Multichannel pipet, 2 Sin-gle
Manual Channel Pipets
 Bacteriology—Sanyo CO2 Incubator, CO2 Gas Analyzer, Bruker Maldi Biotyper with computer/
printer, Mini Spin Centrifuge
 Sample/Receiving—Walk-in Cooler
Northwestern Laboratory
 Installed new Ethernet
Griffin Laboratory
 Centrifuge
Western Laboratory
 4 Eyewash Stations, 2 Safety Showers
2015-2016 Test Procedures Added
 Rollins Lab Molecular Diagnostics—Seneca virus A (Seneca Valley Virus)
Professional Development and Outreach
FNAME MEETING CITY
BEG
DATE
Dr. Allison Boone 2015 USAHA/AAVLD Annual Meeting Providence, RI 10/22/2015
Tina Buffington 2015 USAHA/AAVLD Annual Meeting Providence, RI 10/21/2015
Tina Buffington AAVLD Accreditation Com. Audit Pool Las Vegas, NV 2/03/2016
Dr. David Drum 2015 Univ. Tenn December Conference Knoxville, TN 12/01/2015
Dr. David Drum Outpatient Medicine Conference Athens, GA 12/05/2015
Dr. Jennifer Haugland 2015 USAHA/AAVLD Annual Meeting Providence, RI 10/22/2015
Dr. Jennifer Haugland 41st Annual Eastern Fish Health Wkshp Atlantic Beach, NC 4/11/2016
Ghazala Jawad 2015 USAHA/AAVLD Annual Meeting Providence, RI 10/22/2015
Dr. Jessica Kees 42nd Annual Gross Pathology Review Knoxville, TN 7/12/2015
Dr. Richard Mock 2015 USAHA/AAVLD Annual Meeting Providence, RI 10/22/2015
Dr. Richard Mock 3rd Annual USALIMS User Group Symp Reynoldsburg, OH 4/04/2016
Dr. Richard Oliver 42nd Annual Gross Pathology Review Knoxville, TN 7/12/2015
Dr. Karen Post 2015 USAHA/AAVLD Annual Meeting Providence, RI 10/22/2015
Dr. Reginald Ridenhour NC Turkey Days Wilmington, NC 9/29/2015
Dr. Reginald Ridenhour NC Veterinary Conference Raleigh, NC 11/05/2015
Dr. Stacy Robinson NC Veterinary Conference Raleigh, NC 11/06/2015
Dr. James Trybus 2015 USAHA/AAVLD Annual Meeting Providence, RI 10/22/2015
Dr. James Trybus AAVLD Accreditation Com. Audit Pool Las Vegas, NV 2/03/2016
Dr. Alison Tucker 2015 ACVP/ASVC/STP Annual Meeting Minneapolis, MN 10/17/2015
Talley Ouro 3rd Annual USALIMS User Group Meeting Reynoldsburg, OH 4/04/2016
Joshua Shepherd 3rd Annual USALIMS User Group Meeting Reynoldsburg, OH 4/04/2016
11
PROFICIENCY TESTING
All four laboratories of the NCVDLS participate in proficiency testing from outside agencies. In addition to
these external proficiency tests, laboratory sections participate in internal testing.
Blind Sample Tests are administered by section supervisors to random technologists. Samples have “dummy”
External Proficiency Testing Test Laboratory Section Administering
Agency
Internal Bacteriology QA Survey Bacterial culture identification Rollins Lab Bacteriology AAVLD
Salmonella Group D Environmental culture Rollins Lab Bacteriology NPIP
Southern Bug Exchange Inter-
Laboratory Comparison Program
Bacterial culture identification Rollins Lab Bacteriology Consortium of
Southern VDLS
Taylorella equigenitalis Bacterial identification Rollins Lab Bacteriology NVSL
Leptospirosis IHC Panel (Ring Test) Rollins Lab IHC AAVLD/NVSL
Neospora Caninum Ring Test Rollins Lab IHC AAVLD/NVSL
Avian Influenza Matrix Primer/ Probe Sets & H5 Pri-mer/
Probe Sets, H5 & H7 PCR
Rollins Lab Molecular NAHLN
Avian Paramyxovirus -1 END/NDV for Matrix and Fusion
Primer/ Probe Sets
Rollins Lab Molecular NAHLN
Swine Influenza Virus Rt-PCR Rollins Lab Molecular NAHLN
CSF/FMD Rt-PCR Rollins Lab Molecular NAHLN
MG & MS PCR PCR Rollins Lab Molecular NPIP
Brucellosis BAPA, Card Rollins Lab Serology NVSL
Anaplasmosis c-ELISA Rollins Lab Serology NVSL
Johne's Disease ELISA Rollins Lab Serology NVSL
Leptospirosis MAT Rollins Lab Serology NVSL
MG & MS Serology Plate, ELISA & HI Rollins Lab Serology NPIP
Avian Influenza AGID Rollins Lab Virology NVSL
Bovine Leukosis Virus ELISA Rollins Lab Virology NVSL
Pseudorabies ELISA (G1 & GB), Latex Rollins Lab Virology NVSL
Bluetongue ELISA Rollins Lab Virology NVSL
Equine Infectious Anemia AGID Rollins Lab Virology NVSL
Salmonella Group D Environmental culture Griffin Lab Bacteriology NPIP
MG & MS Serology Plate, ELISA & HI Griffin Lab Serology NPIP
Avian Influenza AGID Griffin Lab Virology NVSL
Brucellosis BAPA, Card Western Lab Serology NVSL
Equine Infectious Anemia AGID Western Lab Virology NVSL
Avian Influenza AGID Northwestern Lab Serology NVSL
MG & MS Serology Plate, ELISA Northwestern Lab Serology NPIP
African Swine Fever & Rinder-pest
Virus
PCR Rollins Lab Molecular NAHLN
West Nile Virus Panel—Equine IgM Capture ELISA Rollins Lab Virology NVSL
PED and PDCoV PCR Rollins Lab Molecular NAHLN
12
NCVDLS 2015-2016 *Accession Information
*Accessions may contain more than one test , discipline, and sample.
Avian 1,240,808 93.14%
Bovine 15,755 1.18%
Camelid 1,009 0.07%
Canine 31,711 2.38%
Caprine 7,960 0.60%
Equine 15,187 1.14%
Feline 7,293 0.55%
Fish 204 0.02%
Miscellaneous 2,457 0.18%
Ovine 1,373 0.10%
Porcine 8,511 0.64%
TOTAL 1,332,268 100.00%
Total Test Count by Animal Species
Fiscal Year Comparison of Accessions
FY 14/15 to FY 15/16
2014-2015 2015-2016 % Increase
Rollins 27,586 30,101 9.12%
Western 1,298 1,289 - 0.7%
Northwestern 5,010 4,709 -6.01%
Griffin 3,589 3,495 -2.62%
Total 37,483 39,594 5.33%
13
NCVDLS Total Test Count and Accession Count Comparison
Year Test Count Accession Count
2012-2013 1,261,919 34,728
2013-2014 1,310,713 36,840
2014-2015 1,327,843 37,483
2015-2016 1,332,317 39,594
NCVDLS Test Count Comparison by Laboratory Discipline
Laboratory Discipline 2012-2013 2013-2014 2014-2015
Bacteriology 17,647 15,843 15,277
Cytology 1,440 1,601 1,805
Histopathology 40,940 43,017 44,920
Immunohistochemistry 690 594 792
Molecular Diagnostics 11,689 16,125 15,302
Mycology 245 233 252
Necropsy 7,337 7,844 7,332
Outsource 1,419 1,299 1,270
Parasitology 2,801 3,040 3,047
Serology 886,698 935,650 940,244
Virology 291,023 285,543 297,602
TOTAL 1,261,929 1,310,789 1,327,843
2015-2016
15,046
2,037
46,051
794
16,724
230
6,476
1,218
3,053
932,326
308,312
1,332,317
14
Top Twenty-Five Tests 2015-2016 2014-2015
Mycoplasma synoviae (ELISA) 381,650 345,716
Mycoplasma gallisepticum (ELISA) 381,397 348,352
Avian Influenza (AGID) 140,933 139,647
Avian Influenza (ELISA ) 101,900 82,011
Mycoplasma meleagridis (ELISA) 53,280 47,522
Mycoplasma gallisepticum (Plate) 44,604 84,925
Mycoplasma synoviae (Plate) 44,564 85,082
Histopathology H & E 44,006 42,213
REO (ELISA) 11,604 12,520
Salmonella pullorum (Plate) 11,400 10,800
Equine Infectious Anemia (AGID) 10,563 10,531
Infectious Bursal Disease (ELISA) 10,265 12,848
Infectious Bronchitis Virus (ELISA) 9,751 12,178
Avian Paramyxovirus-1 (APMV-1) (ELISA) 9,720 11,951
Avian Influenza Matrix RRT-PCR 8,271 6,643
Aerobic Culture 7,728 7,629
Brucella abortus (BAPA) (Plate) 4,399 4,780
Chicken Anemia Virus 1:10 (CAV) (ELISA) 3,534 3,391
Salmonella pullorum (Tube) 3,226 3,736
Gross Exam Poultry 3,168 4,032
Pseudorabies Virus gB (ELISA) 3,030 2,411
Mycoplasma gallisepticum PCR 2,479 1,731
Mycoplasma synoviae PCR 2,478 1,762
NPIP Salmonella Culture 2,426 3,007
Antimicrobial Susceptibility 2,171 2,118
2013-2014
317,526
317,774
137,912
80,209
43,295
115,150
115,098
40,982
10,263
12,300
10,868
10,374
9,410
9,349
6,994
8,014
4,821
3,165
3,439
4,611
5,113
1,718
1,684
2,655
2,189
Accession Counts Comparison
Lab 2012-2013 2013-2014 2014-2015
Rollins Lab 25,239 27,146 27,586
Griffin Lab 3,629 3,511 3,589
Northwestern Lab 4,755 4,914 5,010
Western Lab 1,105 1,269 1,298
34,728 36,840 37,483
Test Counts Comparison
Lab 2012-2013 2013-2014 2014-2015
Rollins Lab 516,266 511,654 517,174
Griffin Lab 184,920 210,484 222,132
Northwestern Lab 558,693 586,088 586,046
Western Lab 2,050 2,563 2,491
1,261,929 1,310,789 1,327,843
2015-2016
30,101
3,495
4,709
1,289
39,594
2015-2016
542,270
224,874
562,254
2,919
1,332,317