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2016-2017 North Carolina Department of Agriculture & Consumer Services Veterinary Division Steven W. Troxler, Commissioner of Agriculture R. Douglas Meckes, DVM, State Veterinarian James Trybus, DVM, DACVP, Director of Laboratories NCVDLS Annual Report Volume 6 1/31/2018 North Carolina Veterinary Diagnostic Laboratory System ii page intentionally left blank iii Table of Contents Page 2 Mission Statement/Message from the Director 3-6 Facilities and Laboratory Personnel 7-8 External Laboratory Advisory Committee 9 Budget and Facility Information 10 Improvements, New Equipment & New Test Procedures 11 Professional Development and Outreach 12 Proficiency Testing 13-15 NCVDLS Accession Information Rollins Animal Disease Diagnostic Laboratory 2101 Blue Ridge Road Raleigh, NC 27607 (919) 733-3986 Western Animal Disease Diagnostic Laboratory 785 Airport Road Fletcher, NC 28732 (828) 684-8188 Northwestern Animal Disease Diagnostic Laboratory 1689 N. Bridge St. Elkin, NC 28621 (336) 526-2499 Griffin Animal Disease Diagnostic Laboratory 401 Quarry Rd. Monroe, NC 28112 (704) 289-6448 NCVDLS Facilities page intentionally left blank 2 On an annual basis, we assemble summaries of the activities within the North Carolina Veterinary Diagnostic Laboratory System (NCVDLS). The information contained within is for the period between July 1, 2016 and June 30, 2017. Data from all four laboratories comprising our system are included. Services have been on the increase, with an escalation of 4.09% overall in laboratory accessions when compared to the previous fiscal year. We hope you find this information to be both interesting and useful. Our goal, as always, is to provide clients with accurate and timely service to meet our Mission, as stated above. Please do not hesitate to contact me about this annual report, if you have questions. Respectfully yours, James Trybus, DVM, D-ACVP NCVDLS Mission Statement It is the mission of the North Carolina Veterinary Diagnostic Laboratory System to provide veterinarians, the animal industry, and the citizens of North Carolina with accurate and timely laboratory support services in order to diagnose, conduct surveillance, and assist in responding to and preventing animal disease. Both protection of public health and the food supply are important components of this mission. 3 NCVDLS Facilities and Laboratory Personnel Rollins Animal Disease Diagnostic Laboratory 1031 Mail Service Center Raleigh, NC 27699-1031 Phone: (919)733-3986 Fax: (919)733-0454 Director of Laboratories James Trybus DVM, D-ACVP Assistant Director Richard Mock, PhD Administrative Staff Kristi Watson Administrative Officer I, Section Supervisor Mechelle Johnson Administrative Assistant I Rebecca Boone Processing Assistant IV Carol Garbarino Processing Assistant IV Dominic Gonzalez Medical Laboratory Assistant II Heather McLean Processing Assistant V Cindy Orlosky Processing Assistant V Brandy Rodgers Processing Assistant IV Dell Weaver Medical Laboratory Assistant II Vacant Processing Assistant III Bacteriology Elizabeth Ortiz, BS Medical Laboratory Supervisor I, Section Supervisor Melissa Cox, MS Medical Laboratory Technologist I Megan Flowers, BS Medical Laboratory Technician II Lee Goldbach, MS Medical Laboratory Technologist I Kelly Hodges, BS Medical Laboratory Technician II Richard Koch Medical Laboratory Technologist I Jennifer Nelson, BS Medical Laboratory Technologist I Jeri Smart, BS, Medical Laboratory Technologist I Laura Tweed Medical Laboratory Technologist I Building Maintenance Stephen Szymczyk Maintenance Mechanic V, Section Supervisor Joey Stephenson Maintenance Mechanic IV Information Technology Josh Shepherd Business and Technology Applications Analyst, Section Supervisor Talley Ouro Business and Technology Applications Analyst Molecular Diagnostics Beverly Wood, BS, MT-ASCP Laboratory Medical Specialist, Section Supervisor Chad Menard, BS Medical Laboratory Technologist III Alla Manakhova, BS Medical Laboratory Technologist II Haley Sutton, BS Medical Laboratory Technologist II Ashlee Zack, BS, Medical Laboratory Technologist II Avian Pathologist Tahseen Abdul Aziz, DVM, MS, PhD, D-ACPV Anatomic Pathologists Allison Boone, DVM, D-ACVP Steve Rushton, DVM, D-ACVP Alison Tucker, MS, VMD, D-ACVP 4 Diagnosticians Mahogany Caesar, MS, DVM Jennifer Haugland, DVM Stacy Robinson, DVM Necropsy Assistant Andrew Chayne, BS Medical Laboratory Technician II Histopathology Cindy Nipper, HT-ACSP Medical Laboratory Technologist III, Section Supervisor Jennifer Burqoyne, BS Medical Laboratory Technician II Judy Liverman Medical Laboratory Technologist I Michelle Price Medical Laboratory Technologist I Immunohistochemistry Vincent Capizzi, AAS Medical Laboratory Technologist II Quality Assurance and Safety Tina Buffington, BS Medical Laboratory Supervisor III, Quality Manager Ghazala Jawad, MS Medical Laboratory Supervisor III, Quality Manager Connie Howen Processing Assistant V Paul Hutter, BS Safety Officer III, Division Safety Officer Serology Kristen Crook, BS, PhD Medical Laboratory Technologist III, Section Supervisor Brandon Brown, BS Medical Laboratory Technologist I Amy Nipper, BS Medical Laboratory Technologist I Aisha Phipps, BS Medical Laboratory Technologist I Virology Neeti Dahal, BS, PhD Medical Laboratory Supervisor III, Section Supervisor Younghee Lee, BS Laboratory Medical Specialist Ashleigh DaGrosa, BS Medical Laboratory Technologist I Jonathan Fox, BS Medical Technician II Christie Lopez-Guzman, MS Medical Laboratory Technologist I Kim Howle, BA, MS Medical Laboratory Technologist III Jenna Hunter, BS Medical Laboratory Technologist I Angela Murphy, BS Medical Laboratory Technologist II Donna Ridenhour, AAS Medical Laboratory Technician II 5 NCVDLS Branch Facilities Griffin Animal Disease Diagnostic Laboratory 401 Quarry Road Monroe, NC 28112 Phone: (704)289-6448 Fax: (704)283-9660 Northwestern Animal Disease Diagnostic Laboratory 1689 N. Bridge St. Elkin, NC 28621 Phone: (336)526-2499 Fax: (336)526-2603 David Ackerman, Resident Director Jessica Kees, DVM Veterinary Diagnostician Terri Jessup, BS Medical Laboratory Technologist II Marcie Sotelo Processing Assistant IV Dana Kelly Veterinary Laboratory Assistant I Amy Sykes, AAS Medical Laboratory Technician II Western Animal Disease Diagnostic Laboratory 785 Airport Road Fletcher, NC 28732 Phone: (828)684-8188 Fax: (828)687-3574 Richard Oliver, DVM Resident Director David Drum, DVM, MS Veterinary Diagnostician Darlene Ayers Processing Assistant IV Lia Barth, BS Medical Laboratory Technician II Wilson Smith Medical Laboratory Assistant II Mary Swanson, DVM Medical Laboratory Technician II Kim Hagans, DVM Resident Director Heather Wyss, DVM Veterinary Diagnostician Sherry Casey Processing Assistant IV Kathy Harwood, AAS Medical Laboratory Technologist I Carrie Lichtenhan, BS Medical Laboratory Technician II Kristie Long Medical Laboratory Technician II Jimmy Walters Medical Laboratory Technician I 6 SERVICE MILESTONES, 2016-2017 Jeri Smart 5 years Wilson Smith 5 years Haley Sutton 5 years Laura Tweed 5 years Dr. Tahseen Aziz 10 years Lee Goldbach 10 years Ghazala Jawad 10 years Kristi Watson 10 years Melissa Cox 15 years Aisha Phipps 15 years Joshua Shepherd 20 years Mechelle Johnson 25 years Darlene Ayers 30 years Dr. Richard Oliver 30 years RETIREMENTS Kim Gooden Rollins Laboratory Serology 12/1/2016 Mary Baker Rollins Laboratory Histology 1/01/2017 Barbara Bright Northwestern Laboratory Serology 1/01/2017 Dr. Reginald Ridenhour Griffin Laboratory Veterinary Diagnostician 1/01/2017 External Laboratory Advisory Committee Members The NCVDLS established an External Laboratory Advisory Committee (ELAC) in 2005. Our initial purpose was to better serve our clients by uniting a diverse group of veterinarians and industry representatives to discuss the laboratory’s offered services, as well as legislation that directly affected our mission. We are appreciative of their time and advice. NAME EMAIL ADDRESS Deborah Johnson, Executive Director NC Pork Council deborah@ncpork.rog Dr. Eric Gonder, Veterinarian Butterball egonder@butterball.com Dr. Allen Cannedy Small Ruminant Veterinarian goat-doc@mymobilevet.com Sue Gray, Executive Director NC Horse Council suegray@nchorsecouncil.com Dr. Randy Jones Mixed Animal Veterinarian Swine Practice, Kinston, NC rjones@livestockvet.com Harry Snelson,, Executive Director American Association of Swine Veterinarians snelson@aasv.org Bryan Blinson, Executive Director NC Cattlemen bryan@nccattle.com Don Butler, Director of Government Relations & Public Affairs Murphy Brown dpbutler@smithfield.com Dr. Becky Tilley, Veterinarian Butterball btilley@butterball.com Dr. Betsy Sigmon, Veterinarian Small Animal Practice btsigmon@aol.com Dr. Chad Smith, Veterinarian Swine Practice, Goldsboro Milling chad.smith@goldsboromilling.com Dr. Paula Cray, Department Head Population Health & Pathobiology, NCSUCVM pjcray@ncsu.edu Dr. Richard Kirkman, Veterinarian Large Animal, Siler City, NC rkirkman@combuy.com 8 External Laboratory Advisory Committee Members cont’ NAME EMAIL ADDRESS Dr. Rich Sharpton, Veterinarian Poultry, Perdue Milling rick.sharpton@perdue.com Dr. Shannon Jennings, Veterinarian Poultry, House of Raeford shannon.jennings@houseofraeford.com Dr. Jennifer Haugland, Veterinarian Diagnostician NCDA&CS, Rollins Animal Disease Diagnostic Labor-atory jennifer.haugland@ncagr.gov Norman Jordan, Past President NC Dairy Producers Association njordan7483@gmail.com Reid Smith, Current President NC Dairy Producers Association reid@linwooddairygroup.com Dr. Douglas Meckes, State Veterinarian NCDA&CS, Veterinary Division doug.meckes@ncagr.gov Dr. James Trybus, Director of Laboratories NCDA&CS, Veterinary Division james.trybus@ncagr.gov Bob Ford, Executive Director Poultry Federation rlford@ncpoultry.org Dr. Glen Almond, Veterinarian NCSUCVM gwalmond@ncsu.edu Dr. Perry Harms, Veterinarian Murphy Brown pharms@smithfield.com Kent Yelverton, Division Director NCDA&CS, Property & Construction kent.yelverton@ncagr.gov Megan Jacob, Laboratory Director NCSUCVM megan_jacob@ncsu.edu Chester Lowder, Livestock Director Farm Bureau chester.lowder@ncfb.org Jan Kelly, Executive Director NC Egg Association egglady@ncegg.org 9 General Funds, Funded Contracts and Cooperative Agreements 2016-2017 NCVDLS General Funds 2016/2017 Recurring Cooperative or Blanket Purchase Agreements USDA-APHIS-VS Blanket Purchase Agreement—$25,440 reimbursement for BSE Surveillance Sample Collection and Testing USDA-APHIS-VS Blanket Purchase Agreement—$6,480 reimbursement for Scrapie Sample Collection and Testing USDA-AHPIS-VS Swine Surveillance Cooperative Agreement—$26,500 reimbursement for surveillance and testing of Classical Swine Fever and PRV samples USDA-NIFA Cooperative Agreement—$326,000 to aid the Food and Agriculture Defense Initiative for surveillance and early detection of animal diseases Budget Summary 2015/2016 2016/2017 Purchased Contractual Services 5321 $184,424 $247,688 Utility/Energy Services 5322 $264,502 $319,407 Repair Services 5323 $105,843 $157,851 Maintenance Agreements 5324 $140,438 $68,977 Rentals/Leases 5325 $5,175 $5,536 Travel & Other Employee Expenses 5327 $43,741 $29,397 Communications & Data 5328 $108,555 $108,215 Other Services 5329 $27,255 $30,405 General Admin Supplies 5331 $28,623 $23,028 Facility and Hardware Supplies 5332 $23,522 $21,050 Vehicle/Equipment Operating Supplies 5333 $1,804 $1,064 Clothing and Recreational Supplies 5335 $1,306 $1,983 Research/Development & Edu Supplies 5337 $1,014,532 $1,214,852, Other Materials & Supplies 5339 $13,354 $39,620 Equipment 5345 $323,782 $48,351 Art, Other Artifacts & Literature 5346 $2,052 $154 Intangible Assets (Computer software) 5347 $44,462 $48,923 Legal, Licenses & Permit Costs 5351 $6,182 $7,383 Other Admin Expenses 5358 $4,357 $2,614 Other Expenses 5359 $8,675 $10,592 Transfer of Appropriations 5389 $0 $0 TOTAL $2,352,584 $2,387,090 Personal Services (Salaries, Benefits, etc.) $5,324,958 $5,468,971 TOTAL $7,677,542 $7,856,061, 10 2016-2017 Physical Improvements/New Equipment Purchases Rollins Laboratory Molecular Diagnostics—Biosecurity Cabinet and Table Serology— Thermo Scientific –20 C Freezer, Thermo Scientific Centrifuge Bacteriology—Tube Rotator Commercial Reverse Osmosis Purity Water System Northwestern Laboratory Autoclave Griffin Laboratory Reciprocating Saw Western Laboratory Hewett Packard model M552 Laser Jet Printer Incinerator Storage Rooms Light Fixtures 2016-2017 Test Procedures Added Rollins Lab Histology—Periodic Acid Schiff with Diatase Stain 11 NAME MEETING CITY BEG DATE Dr. David Ackerman 2016 NC Veterinary Conference Raleigh, NC 11/03/2016 Dr. David Ackerman 2016 Poultry Health Meeting Raleigh, NC 11/11/2016 Dr. Tahseen Abdul-Aziz 2016 Annual American Vet Med Association San Antonio, TX 8/06/2016 Dr. Allison Boone 2016 ACVP & ASVCP Annual Meeting New Orleans, LA 12/02/2016 Tina Buffington 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/12/2016 Dr. Mahogany Caesar 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. Neeti Dahal 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. David Drum Pathology Continuing Education Workshop Athens, GA Dr. David Drum 2016 Henton Veterinary Conference Knoxville, TN 11/29/2016 Dr. Jennifer Haugland 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/16/2016 Ghazala Jawad 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/12/2016 Dr. Jessica Kees 2016 Theriogenology Conference Asheville, NC 9/26/2016 Younghee Lee 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. Richard Mock 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/13/2016 Dr. Richard Oliver 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Talley Ouro Annual USALIMS User Group Symposium Hopkinsville, KY 4/10/2017 Dr. Reginald Ridenhour American Assoc of Bovine Pract Conference Charlotte, NC 9/15/2016 Dr. Reginald Ridenhour 2016 NC Veterinary Conference Raleigh, NC 11/04/2016 Dr. Stacy Robinson 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. Steven Rushton 2016 ACVP & ASVCP Annual Meeting New Orleans, LA 10/02/2016 Joshua Shepherd Annual USALIMS User Group Symposium Hopkinsville, KY 4/10/2017 Dr. Mary Swanson 2016 One Medicine Symposium Raleigh, NC 12/06/2016 Dr. James Trybus 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. Alison Tucker 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/13/2016 Professional Development and Outreach 12 PROFICIENCY TESTING All four NCVLS laboratories participate in proficiency testing from outside agencies. In addi-tion to the external proficiency tests, laboratory sections participate in internal testing. Blind Sample Tests are administered by section supervisors to random technologists. Samples have “dummy”. External Proficiency Testing Test Laboratory Section Administering Agency Internal Bacteriology QA Survey Bacterial culture identification Rollins Lab Bacteriology AAVLD Salmonella Group D Environmental culture Rollins Lab Bacteriology NPIP Southern Bug Exchange Inter-Laboratory Comparison Program Bacterial culture identification Rollins Lab Bacteriology Consortium of Southern VDLS Avian Influenza Matrix Primer/ Probe Sets & H5 Pri-mer/ Probe Sets, H5 & H7 PCR Rollins Lab Molecular NAHLN Avian Paramyxovirus -1 END/NDV for Matrix and Fusion Primer/ Probe Sets Rollins Lab Molecular NAHLN Swine Influenza Virus Rt-PCR Rollins Lab Molecular NAHLN CSF/FMD Rt-PCR Rollins Lab Molecular NAHLN MG & MS PCR PCR Rollins Lab Molecular NPIP African Swine Fever & Rinder-pest Virus PCR Rollins Lab Molecular NAHLN Foor and Mouth Disease FMD (ELISA) Rollins Lab Molecular NAHLN Brucellosis BAPA, Card Rollins Lab Serology NVSL Anaplasmosis c-ELISA Rollins Lab Serology NVSL Johne's Disease ELISA Rollins Lab Serology NVSL Leptospirosis MAT Rollins Lab Serology NVSL MG & MS Serology Plate, ELISA & HI Rollins Lab Serology NPIP Avian Influenza AGID Rollins Lab Virology NVSL Bovine Leukosis Virus ELISA Rollins Lab Virology NVSL Pseudorabies ELISA (G1 & GB), Latex Rollins Lab Virology NVSL Bluetongue ELISA Rollins Lab Virology NVSL Equine Infectious Anemia AGID Rollins Lab Virology NVSL Parallel IHC - Corona V (K-9) Stain Comparison with MSU Rollins Lab IHC MSU Salmonella Group D Environmental culture Griffin Lab Bacteriology NPIP MG & MS Serology Plate, ELISA & HI Griffin Lab Serology NPIP Avian Influenza AGID Griffin Lab Virology NVSL Brucellosis BAPA, Card Western Lab Serology NVSL Equine Infectious Anemia AGID Western Lab Virology NVSL Avian Influenza AGID Northwestern Lab Serology NVSL MG & MS Serology Plate, ELISA Northwestern Lab Serology NPIP 13 NCVDLS 2016-2017 *Accession Information *Accessions may contain more than one test, discipline and sample. Avian 1,189,253 93.16% Bovine 14,595 1.14% Camelid 909 .07% Canine 32,449 2.54% Caprine 7,866 .62% Equine 13,872 1.09% Feline 7,347 .58% Fish 257 .02% Miscellaneous 2,211 .17% Ovine 1,215 .10% Porcine 6,538 .51% TOTAL 1,276,512 100.00% Total Test Count by Animal Species Fiscal Year Comparison of Accessions FY 15/16 to FY 16/17 2015-2016 2016-2017 % Increase Rollins 30,101 32,265 6.71% Western 1,289 1,274 -1.18% Northwestern 4,709 4,579 -2.84% Griffin 3,495 3,163 -10.50% Total 39,594 41,281 4.09% 16% 25% 48% 11% NCVDLS Necropsy Test Count - 6,911 Griffin Animal Disease Diagnostic Laboratory, 1,136 Northwestern Animal Disease Diagnostic Laboratory, 1,748 Rollins Animal Disease Diagnostic Laboratory, 3,288 Western Animal Disease Diagnostic Laboratory, 739 8% 11% 78% 3% 2016-2017 Accession Count for NCVDLS: 41,281 Increase over 2015-2016 of 4.09% Griffin Animal Disease Diagnostic Laboratory, 3,163 Northwestern Animal Disease Diagnostic Laboratory, 4,579 Rollins Animal Disease Diagnostic Laboratory, 32,265 Western Animal Disease Diagnostic Laboratory, 1,274 14 NCVDLS Total Test Count and Accession Count Comparison Year Test Count Accession Count 2013-2014 1,310,793 36,840 2014-2015 1,327,846 37,483 2015-2016 1,332,319 39,594 2016-2017 1,278,459 41,281 NCVDLS Test Count Comparison by Laboratory Discipline Laboratory Discipline 2013-2014 2014-2015 2015-2016 2016-2017 Bacteriology 15,843 15,277 15,046 14,761 Cytology 1,601 1,805 2,027 2,197 Histopathology 43,021 44,923 46,063 44,006 Immunohistochemistry 594 792 794 897 Molecular Diagnostics 16,125 15,302 16,724 17,954 Mycology 233 252 230 251 Necropsy 7,844 7,332 6,476 6,911 Outsource 1,299 1,270 1,218 1,317 Parasitology 3,040 3,047 3,053 3,023 Serology 935,650 940,244 932,376 879,663 Virology 285,543 297,602 308,312 307,479 TOTAL 1,310,793 1,327,846 1,332,319 1,278,459 15 Top Twenty-Five Tests 2015-2016 2014-2015 2016-2017 Mycoplasma synoviae (ELISA) 381,650 346,780 301,513 Mycoplasma gallisepticum (ELISA) 381,397 349,416 301,506 Avian Influenza (AGID) 140,933 139,549 131,537 Avian Influenza (ELISA ) 101,900 81,758 115,587 Mycoplasma gallisepticum (Plate) 44,604 84,926 102,500 Mycoplasma synoviae (Plate) 44,564 85,080 102,011 Mycoplasma meleagridis (ELISA) 53,280 48,112 44,728 Histopathology H & E 44,006 42,141 41,686 Salmonella pullorum (Plate) 11,400 11,400 10,200 Infectious Bursal Disease (ELISA) 10,265 12,990 9,861 REO (ELISA) 11,604 12,659 9,831 Equine Infectious Anemia (AGID) 10,563 10,497 9,704 Avian Influenza Matrix RRT-PCR 8,271 6,675 9,386 Infectious Bronchitis Virus (ELISA) 9,751 12,210 9,065 Avian Paramyxovirus (ELISA) 9,721 11,983 8,825 Aerobic Culture 7,728 7,646 7,313 Brucella abortus (Plate) 4,399 4,780 4,044 Gross Exam Poultry 3,168 4,032 3,657 Chicken Anemia Virus (ELISA) 3,534 3,383 3,472 Mycoplasma synoviae PCR 2,478 1,762 3,470 Mycoplasma gallisepticum PCR 2,479 1,731 3,456 Salmonella pullorum (Tube) 3,226 3,736 2,858 Pseudorabies Virus gB (ELISA) 3,033 2,410 2,548 Antimicrobial Susceptibility 2,171 2,152 2,493 NPIP Salmonella Culture 2,426 3,015 2,307 Accession Counts Comparison Lab 2013-2014 2014-2015 2015-2016 2016-2017 Rollins Lab 27,146 27,586 30,101 32,265 Griffin Lab 3,511 3,589 3,495 3,163 Northwestern Lab 4,914 5,010 4,709 4,579 Western Lab 1,269 1,298 1,289 1,274 36,840 37,483 39,594 41,281 Test Counts Comparison Lab 2013-2014 2014-2015 2015-2016 2016-2017 Rollins Lab 511,658 517,177 542,272 547,927 Griffin Lab 210,484 222,132 224,874 191,296 Northwestern Lab 586,088 586,046 562,254 536,713 Western Lab 2,563 2,491 2,919 2,523 1,310,793 1,327,846 1,332,319 1,278,459 16 page intentionally left blank
Object Description
Description
Title | North Carolina Veterinary Diagnostic Laboratory System annual report |
Other Title | NCVDLS annual report |
Date | 2018-01-31 |
Description | Volume 6 (2016-2017) |
Digital Characteristics-A | 1.16 MB; 19 p. |
Digital Format | application/pdf |
Pres File Name-M | pubs_serial_921301840_ncvdls20162017v6 |
Full Text | 2016-2017 North Carolina Department of Agriculture & Consumer Services Veterinary Division Steven W. Troxler, Commissioner of Agriculture R. Douglas Meckes, DVM, State Veterinarian James Trybus, DVM, DACVP, Director of Laboratories NCVDLS Annual Report Volume 6 1/31/2018 North Carolina Veterinary Diagnostic Laboratory System ii page intentionally left blank iii Table of Contents Page 2 Mission Statement/Message from the Director 3-6 Facilities and Laboratory Personnel 7-8 External Laboratory Advisory Committee 9 Budget and Facility Information 10 Improvements, New Equipment & New Test Procedures 11 Professional Development and Outreach 12 Proficiency Testing 13-15 NCVDLS Accession Information Rollins Animal Disease Diagnostic Laboratory 2101 Blue Ridge Road Raleigh, NC 27607 (919) 733-3986 Western Animal Disease Diagnostic Laboratory 785 Airport Road Fletcher, NC 28732 (828) 684-8188 Northwestern Animal Disease Diagnostic Laboratory 1689 N. Bridge St. Elkin, NC 28621 (336) 526-2499 Griffin Animal Disease Diagnostic Laboratory 401 Quarry Rd. Monroe, NC 28112 (704) 289-6448 NCVDLS Facilities page intentionally left blank 2 On an annual basis, we assemble summaries of the activities within the North Carolina Veterinary Diagnostic Laboratory System (NCVDLS). The information contained within is for the period between July 1, 2016 and June 30, 2017. Data from all four laboratories comprising our system are included. Services have been on the increase, with an escalation of 4.09% overall in laboratory accessions when compared to the previous fiscal year. We hope you find this information to be both interesting and useful. Our goal, as always, is to provide clients with accurate and timely service to meet our Mission, as stated above. Please do not hesitate to contact me about this annual report, if you have questions. Respectfully yours, James Trybus, DVM, D-ACVP NCVDLS Mission Statement It is the mission of the North Carolina Veterinary Diagnostic Laboratory System to provide veterinarians, the animal industry, and the citizens of North Carolina with accurate and timely laboratory support services in order to diagnose, conduct surveillance, and assist in responding to and preventing animal disease. Both protection of public health and the food supply are important components of this mission. 3 NCVDLS Facilities and Laboratory Personnel Rollins Animal Disease Diagnostic Laboratory 1031 Mail Service Center Raleigh, NC 27699-1031 Phone: (919)733-3986 Fax: (919)733-0454 Director of Laboratories James Trybus DVM, D-ACVP Assistant Director Richard Mock, PhD Administrative Staff Kristi Watson Administrative Officer I, Section Supervisor Mechelle Johnson Administrative Assistant I Rebecca Boone Processing Assistant IV Carol Garbarino Processing Assistant IV Dominic Gonzalez Medical Laboratory Assistant II Heather McLean Processing Assistant V Cindy Orlosky Processing Assistant V Brandy Rodgers Processing Assistant IV Dell Weaver Medical Laboratory Assistant II Vacant Processing Assistant III Bacteriology Elizabeth Ortiz, BS Medical Laboratory Supervisor I, Section Supervisor Melissa Cox, MS Medical Laboratory Technologist I Megan Flowers, BS Medical Laboratory Technician II Lee Goldbach, MS Medical Laboratory Technologist I Kelly Hodges, BS Medical Laboratory Technician II Richard Koch Medical Laboratory Technologist I Jennifer Nelson, BS Medical Laboratory Technologist I Jeri Smart, BS, Medical Laboratory Technologist I Laura Tweed Medical Laboratory Technologist I Building Maintenance Stephen Szymczyk Maintenance Mechanic V, Section Supervisor Joey Stephenson Maintenance Mechanic IV Information Technology Josh Shepherd Business and Technology Applications Analyst, Section Supervisor Talley Ouro Business and Technology Applications Analyst Molecular Diagnostics Beverly Wood, BS, MT-ASCP Laboratory Medical Specialist, Section Supervisor Chad Menard, BS Medical Laboratory Technologist III Alla Manakhova, BS Medical Laboratory Technologist II Haley Sutton, BS Medical Laboratory Technologist II Ashlee Zack, BS, Medical Laboratory Technologist II Avian Pathologist Tahseen Abdul Aziz, DVM, MS, PhD, D-ACPV Anatomic Pathologists Allison Boone, DVM, D-ACVP Steve Rushton, DVM, D-ACVP Alison Tucker, MS, VMD, D-ACVP 4 Diagnosticians Mahogany Caesar, MS, DVM Jennifer Haugland, DVM Stacy Robinson, DVM Necropsy Assistant Andrew Chayne, BS Medical Laboratory Technician II Histopathology Cindy Nipper, HT-ACSP Medical Laboratory Technologist III, Section Supervisor Jennifer Burqoyne, BS Medical Laboratory Technician II Judy Liverman Medical Laboratory Technologist I Michelle Price Medical Laboratory Technologist I Immunohistochemistry Vincent Capizzi, AAS Medical Laboratory Technologist II Quality Assurance and Safety Tina Buffington, BS Medical Laboratory Supervisor III, Quality Manager Ghazala Jawad, MS Medical Laboratory Supervisor III, Quality Manager Connie Howen Processing Assistant V Paul Hutter, BS Safety Officer III, Division Safety Officer Serology Kristen Crook, BS, PhD Medical Laboratory Technologist III, Section Supervisor Brandon Brown, BS Medical Laboratory Technologist I Amy Nipper, BS Medical Laboratory Technologist I Aisha Phipps, BS Medical Laboratory Technologist I Virology Neeti Dahal, BS, PhD Medical Laboratory Supervisor III, Section Supervisor Younghee Lee, BS Laboratory Medical Specialist Ashleigh DaGrosa, BS Medical Laboratory Technologist I Jonathan Fox, BS Medical Technician II Christie Lopez-Guzman, MS Medical Laboratory Technologist I Kim Howle, BA, MS Medical Laboratory Technologist III Jenna Hunter, BS Medical Laboratory Technologist I Angela Murphy, BS Medical Laboratory Technologist II Donna Ridenhour, AAS Medical Laboratory Technician II 5 NCVDLS Branch Facilities Griffin Animal Disease Diagnostic Laboratory 401 Quarry Road Monroe, NC 28112 Phone: (704)289-6448 Fax: (704)283-9660 Northwestern Animal Disease Diagnostic Laboratory 1689 N. Bridge St. Elkin, NC 28621 Phone: (336)526-2499 Fax: (336)526-2603 David Ackerman, Resident Director Jessica Kees, DVM Veterinary Diagnostician Terri Jessup, BS Medical Laboratory Technologist II Marcie Sotelo Processing Assistant IV Dana Kelly Veterinary Laboratory Assistant I Amy Sykes, AAS Medical Laboratory Technician II Western Animal Disease Diagnostic Laboratory 785 Airport Road Fletcher, NC 28732 Phone: (828)684-8188 Fax: (828)687-3574 Richard Oliver, DVM Resident Director David Drum, DVM, MS Veterinary Diagnostician Darlene Ayers Processing Assistant IV Lia Barth, BS Medical Laboratory Technician II Wilson Smith Medical Laboratory Assistant II Mary Swanson, DVM Medical Laboratory Technician II Kim Hagans, DVM Resident Director Heather Wyss, DVM Veterinary Diagnostician Sherry Casey Processing Assistant IV Kathy Harwood, AAS Medical Laboratory Technologist I Carrie Lichtenhan, BS Medical Laboratory Technician II Kristie Long Medical Laboratory Technician II Jimmy Walters Medical Laboratory Technician I 6 SERVICE MILESTONES, 2016-2017 Jeri Smart 5 years Wilson Smith 5 years Haley Sutton 5 years Laura Tweed 5 years Dr. Tahseen Aziz 10 years Lee Goldbach 10 years Ghazala Jawad 10 years Kristi Watson 10 years Melissa Cox 15 years Aisha Phipps 15 years Joshua Shepherd 20 years Mechelle Johnson 25 years Darlene Ayers 30 years Dr. Richard Oliver 30 years RETIREMENTS Kim Gooden Rollins Laboratory Serology 12/1/2016 Mary Baker Rollins Laboratory Histology 1/01/2017 Barbara Bright Northwestern Laboratory Serology 1/01/2017 Dr. Reginald Ridenhour Griffin Laboratory Veterinary Diagnostician 1/01/2017 External Laboratory Advisory Committee Members The NCVDLS established an External Laboratory Advisory Committee (ELAC) in 2005. Our initial purpose was to better serve our clients by uniting a diverse group of veterinarians and industry representatives to discuss the laboratory’s offered services, as well as legislation that directly affected our mission. We are appreciative of their time and advice. NAME EMAIL ADDRESS Deborah Johnson, Executive Director NC Pork Council deborah@ncpork.rog Dr. Eric Gonder, Veterinarian Butterball egonder@butterball.com Dr. Allen Cannedy Small Ruminant Veterinarian goat-doc@mymobilevet.com Sue Gray, Executive Director NC Horse Council suegray@nchorsecouncil.com Dr. Randy Jones Mixed Animal Veterinarian Swine Practice, Kinston, NC rjones@livestockvet.com Harry Snelson,, Executive Director American Association of Swine Veterinarians snelson@aasv.org Bryan Blinson, Executive Director NC Cattlemen bryan@nccattle.com Don Butler, Director of Government Relations & Public Affairs Murphy Brown dpbutler@smithfield.com Dr. Becky Tilley, Veterinarian Butterball btilley@butterball.com Dr. Betsy Sigmon, Veterinarian Small Animal Practice btsigmon@aol.com Dr. Chad Smith, Veterinarian Swine Practice, Goldsboro Milling chad.smith@goldsboromilling.com Dr. Paula Cray, Department Head Population Health & Pathobiology, NCSUCVM pjcray@ncsu.edu Dr. Richard Kirkman, Veterinarian Large Animal, Siler City, NC rkirkman@combuy.com 8 External Laboratory Advisory Committee Members cont’ NAME EMAIL ADDRESS Dr. Rich Sharpton, Veterinarian Poultry, Perdue Milling rick.sharpton@perdue.com Dr. Shannon Jennings, Veterinarian Poultry, House of Raeford shannon.jennings@houseofraeford.com Dr. Jennifer Haugland, Veterinarian Diagnostician NCDA&CS, Rollins Animal Disease Diagnostic Labor-atory jennifer.haugland@ncagr.gov Norman Jordan, Past President NC Dairy Producers Association njordan7483@gmail.com Reid Smith, Current President NC Dairy Producers Association reid@linwooddairygroup.com Dr. Douglas Meckes, State Veterinarian NCDA&CS, Veterinary Division doug.meckes@ncagr.gov Dr. James Trybus, Director of Laboratories NCDA&CS, Veterinary Division james.trybus@ncagr.gov Bob Ford, Executive Director Poultry Federation rlford@ncpoultry.org Dr. Glen Almond, Veterinarian NCSUCVM gwalmond@ncsu.edu Dr. Perry Harms, Veterinarian Murphy Brown pharms@smithfield.com Kent Yelverton, Division Director NCDA&CS, Property & Construction kent.yelverton@ncagr.gov Megan Jacob, Laboratory Director NCSUCVM megan_jacob@ncsu.edu Chester Lowder, Livestock Director Farm Bureau chester.lowder@ncfb.org Jan Kelly, Executive Director NC Egg Association egglady@ncegg.org 9 General Funds, Funded Contracts and Cooperative Agreements 2016-2017 NCVDLS General Funds 2016/2017 Recurring Cooperative or Blanket Purchase Agreements USDA-APHIS-VS Blanket Purchase Agreement—$25,440 reimbursement for BSE Surveillance Sample Collection and Testing USDA-APHIS-VS Blanket Purchase Agreement—$6,480 reimbursement for Scrapie Sample Collection and Testing USDA-AHPIS-VS Swine Surveillance Cooperative Agreement—$26,500 reimbursement for surveillance and testing of Classical Swine Fever and PRV samples USDA-NIFA Cooperative Agreement—$326,000 to aid the Food and Agriculture Defense Initiative for surveillance and early detection of animal diseases Budget Summary 2015/2016 2016/2017 Purchased Contractual Services 5321 $184,424 $247,688 Utility/Energy Services 5322 $264,502 $319,407 Repair Services 5323 $105,843 $157,851 Maintenance Agreements 5324 $140,438 $68,977 Rentals/Leases 5325 $5,175 $5,536 Travel & Other Employee Expenses 5327 $43,741 $29,397 Communications & Data 5328 $108,555 $108,215 Other Services 5329 $27,255 $30,405 General Admin Supplies 5331 $28,623 $23,028 Facility and Hardware Supplies 5332 $23,522 $21,050 Vehicle/Equipment Operating Supplies 5333 $1,804 $1,064 Clothing and Recreational Supplies 5335 $1,306 $1,983 Research/Development & Edu Supplies 5337 $1,014,532 $1,214,852, Other Materials & Supplies 5339 $13,354 $39,620 Equipment 5345 $323,782 $48,351 Art, Other Artifacts & Literature 5346 $2,052 $154 Intangible Assets (Computer software) 5347 $44,462 $48,923 Legal, Licenses & Permit Costs 5351 $6,182 $7,383 Other Admin Expenses 5358 $4,357 $2,614 Other Expenses 5359 $8,675 $10,592 Transfer of Appropriations 5389 $0 $0 TOTAL $2,352,584 $2,387,090 Personal Services (Salaries, Benefits, etc.) $5,324,958 $5,468,971 TOTAL $7,677,542 $7,856,061, 10 2016-2017 Physical Improvements/New Equipment Purchases Rollins Laboratory Molecular Diagnostics—Biosecurity Cabinet and Table Serology— Thermo Scientific –20 C Freezer, Thermo Scientific Centrifuge Bacteriology—Tube Rotator Commercial Reverse Osmosis Purity Water System Northwestern Laboratory Autoclave Griffin Laboratory Reciprocating Saw Western Laboratory Hewett Packard model M552 Laser Jet Printer Incinerator Storage Rooms Light Fixtures 2016-2017 Test Procedures Added Rollins Lab Histology—Periodic Acid Schiff with Diatase Stain 11 NAME MEETING CITY BEG DATE Dr. David Ackerman 2016 NC Veterinary Conference Raleigh, NC 11/03/2016 Dr. David Ackerman 2016 Poultry Health Meeting Raleigh, NC 11/11/2016 Dr. Tahseen Abdul-Aziz 2016 Annual American Vet Med Association San Antonio, TX 8/06/2016 Dr. Allison Boone 2016 ACVP & ASVCP Annual Meeting New Orleans, LA 12/02/2016 Tina Buffington 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/12/2016 Dr. Mahogany Caesar 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. Neeti Dahal 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. David Drum Pathology Continuing Education Workshop Athens, GA Dr. David Drum 2016 Henton Veterinary Conference Knoxville, TN 11/29/2016 Dr. Jennifer Haugland 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/16/2016 Ghazala Jawad 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/12/2016 Dr. Jessica Kees 2016 Theriogenology Conference Asheville, NC 9/26/2016 Younghee Lee 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. Richard Mock 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/13/2016 Dr. Richard Oliver 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Talley Ouro Annual USALIMS User Group Symposium Hopkinsville, KY 4/10/2017 Dr. Reginald Ridenhour American Assoc of Bovine Pract Conference Charlotte, NC 9/15/2016 Dr. Reginald Ridenhour 2016 NC Veterinary Conference Raleigh, NC 11/04/2016 Dr. Stacy Robinson 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. Steven Rushton 2016 ACVP & ASVCP Annual Meeting New Orleans, LA 10/02/2016 Joshua Shepherd Annual USALIMS User Group Symposium Hopkinsville, KY 4/10/2017 Dr. Mary Swanson 2016 One Medicine Symposium Raleigh, NC 12/06/2016 Dr. James Trybus 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/14/2016 Dr. Alison Tucker 2016 USAHA/AAVLD Annual Meeting Greensboro, NC 10/13/2016 Professional Development and Outreach 12 PROFICIENCY TESTING All four NCVLS laboratories participate in proficiency testing from outside agencies. In addi-tion to the external proficiency tests, laboratory sections participate in internal testing. Blind Sample Tests are administered by section supervisors to random technologists. Samples have “dummy”. External Proficiency Testing Test Laboratory Section Administering Agency Internal Bacteriology QA Survey Bacterial culture identification Rollins Lab Bacteriology AAVLD Salmonella Group D Environmental culture Rollins Lab Bacteriology NPIP Southern Bug Exchange Inter-Laboratory Comparison Program Bacterial culture identification Rollins Lab Bacteriology Consortium of Southern VDLS Avian Influenza Matrix Primer/ Probe Sets & H5 Pri-mer/ Probe Sets, H5 & H7 PCR Rollins Lab Molecular NAHLN Avian Paramyxovirus -1 END/NDV for Matrix and Fusion Primer/ Probe Sets Rollins Lab Molecular NAHLN Swine Influenza Virus Rt-PCR Rollins Lab Molecular NAHLN CSF/FMD Rt-PCR Rollins Lab Molecular NAHLN MG & MS PCR PCR Rollins Lab Molecular NPIP African Swine Fever & Rinder-pest Virus PCR Rollins Lab Molecular NAHLN Foor and Mouth Disease FMD (ELISA) Rollins Lab Molecular NAHLN Brucellosis BAPA, Card Rollins Lab Serology NVSL Anaplasmosis c-ELISA Rollins Lab Serology NVSL Johne's Disease ELISA Rollins Lab Serology NVSL Leptospirosis MAT Rollins Lab Serology NVSL MG & MS Serology Plate, ELISA & HI Rollins Lab Serology NPIP Avian Influenza AGID Rollins Lab Virology NVSL Bovine Leukosis Virus ELISA Rollins Lab Virology NVSL Pseudorabies ELISA (G1 & GB), Latex Rollins Lab Virology NVSL Bluetongue ELISA Rollins Lab Virology NVSL Equine Infectious Anemia AGID Rollins Lab Virology NVSL Parallel IHC - Corona V (K-9) Stain Comparison with MSU Rollins Lab IHC MSU Salmonella Group D Environmental culture Griffin Lab Bacteriology NPIP MG & MS Serology Plate, ELISA & HI Griffin Lab Serology NPIP Avian Influenza AGID Griffin Lab Virology NVSL Brucellosis BAPA, Card Western Lab Serology NVSL Equine Infectious Anemia AGID Western Lab Virology NVSL Avian Influenza AGID Northwestern Lab Serology NVSL MG & MS Serology Plate, ELISA Northwestern Lab Serology NPIP 13 NCVDLS 2016-2017 *Accession Information *Accessions may contain more than one test, discipline and sample. Avian 1,189,253 93.16% Bovine 14,595 1.14% Camelid 909 .07% Canine 32,449 2.54% Caprine 7,866 .62% Equine 13,872 1.09% Feline 7,347 .58% Fish 257 .02% Miscellaneous 2,211 .17% Ovine 1,215 .10% Porcine 6,538 .51% TOTAL 1,276,512 100.00% Total Test Count by Animal Species Fiscal Year Comparison of Accessions FY 15/16 to FY 16/17 2015-2016 2016-2017 % Increase Rollins 30,101 32,265 6.71% Western 1,289 1,274 -1.18% Northwestern 4,709 4,579 -2.84% Griffin 3,495 3,163 -10.50% Total 39,594 41,281 4.09% 16% 25% 48% 11% NCVDLS Necropsy Test Count - 6,911 Griffin Animal Disease Diagnostic Laboratory, 1,136 Northwestern Animal Disease Diagnostic Laboratory, 1,748 Rollins Animal Disease Diagnostic Laboratory, 3,288 Western Animal Disease Diagnostic Laboratory, 739 8% 11% 78% 3% 2016-2017 Accession Count for NCVDLS: 41,281 Increase over 2015-2016 of 4.09% Griffin Animal Disease Diagnostic Laboratory, 3,163 Northwestern Animal Disease Diagnostic Laboratory, 4,579 Rollins Animal Disease Diagnostic Laboratory, 32,265 Western Animal Disease Diagnostic Laboratory, 1,274 14 NCVDLS Total Test Count and Accession Count Comparison Year Test Count Accession Count 2013-2014 1,310,793 36,840 2014-2015 1,327,846 37,483 2015-2016 1,332,319 39,594 2016-2017 1,278,459 41,281 NCVDLS Test Count Comparison by Laboratory Discipline Laboratory Discipline 2013-2014 2014-2015 2015-2016 2016-2017 Bacteriology 15,843 15,277 15,046 14,761 Cytology 1,601 1,805 2,027 2,197 Histopathology 43,021 44,923 46,063 44,006 Immunohistochemistry 594 792 794 897 Molecular Diagnostics 16,125 15,302 16,724 17,954 Mycology 233 252 230 251 Necropsy 7,844 7,332 6,476 6,911 Outsource 1,299 1,270 1,218 1,317 Parasitology 3,040 3,047 3,053 3,023 Serology 935,650 940,244 932,376 879,663 Virology 285,543 297,602 308,312 307,479 TOTAL 1,310,793 1,327,846 1,332,319 1,278,459 15 Top Twenty-Five Tests 2015-2016 2014-2015 2016-2017 Mycoplasma synoviae (ELISA) 381,650 346,780 301,513 Mycoplasma gallisepticum (ELISA) 381,397 349,416 301,506 Avian Influenza (AGID) 140,933 139,549 131,537 Avian Influenza (ELISA ) 101,900 81,758 115,587 Mycoplasma gallisepticum (Plate) 44,604 84,926 102,500 Mycoplasma synoviae (Plate) 44,564 85,080 102,011 Mycoplasma meleagridis (ELISA) 53,280 48,112 44,728 Histopathology H & E 44,006 42,141 41,686 Salmonella pullorum (Plate) 11,400 11,400 10,200 Infectious Bursal Disease (ELISA) 10,265 12,990 9,861 REO (ELISA) 11,604 12,659 9,831 Equine Infectious Anemia (AGID) 10,563 10,497 9,704 Avian Influenza Matrix RRT-PCR 8,271 6,675 9,386 Infectious Bronchitis Virus (ELISA) 9,751 12,210 9,065 Avian Paramyxovirus (ELISA) 9,721 11,983 8,825 Aerobic Culture 7,728 7,646 7,313 Brucella abortus (Plate) 4,399 4,780 4,044 Gross Exam Poultry 3,168 4,032 3,657 Chicken Anemia Virus (ELISA) 3,534 3,383 3,472 Mycoplasma synoviae PCR 2,478 1,762 3,470 Mycoplasma gallisepticum PCR 2,479 1,731 3,456 Salmonella pullorum (Tube) 3,226 3,736 2,858 Pseudorabies Virus gB (ELISA) 3,033 2,410 2,548 Antimicrobial Susceptibility 2,171 2,152 2,493 NPIP Salmonella Culture 2,426 3,015 2,307 Accession Counts Comparison Lab 2013-2014 2014-2015 2015-2016 2016-2017 Rollins Lab 27,146 27,586 30,101 32,265 Griffin Lab 3,511 3,589 3,495 3,163 Northwestern Lab 4,914 5,010 4,709 4,579 Western Lab 1,269 1,298 1,289 1,274 36,840 37,483 39,594 41,281 Test Counts Comparison Lab 2013-2014 2014-2015 2015-2016 2016-2017 Rollins Lab 511,658 517,177 542,272 547,927 Griffin Lab 210,484 222,132 224,874 191,296 Northwestern Lab 586,088 586,046 562,254 536,713 Western Lab 2,563 2,491 2,919 2,523 1,310,793 1,327,846 1,332,319 1,278,459 16 page intentionally left blank |
OCLC number | 921301840 |